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MALDI imaging : ウィキペディア英語版
MALDI imaging

MALDI imaging mass spectrometry (MALDI-IMS) is the use of matrix-assisted laser desorption ionization as a mass spectrometry imaging technique in which the sample, often a thin tissue section, is moved in two dimensions while the mass spectrum is recorded. Advantages, like measuring the distribution of a large amount of analytes at one time without destroying the sample, make it a useful method in tissue-based study.〔Walch, A., Rauser, S., Deininger, S. O., & Hofler, H. (2008). MALDI imaging mass spectrometry for direct tissue analysis: a new frontier for molecular histology. Histochemistry and cell biology, 130(3), 421-434. (doi:10.1007/s00418-008-0469-9 )〕
== Sample preparation ==

Sample preparation is a critical step in imaging spectroscopy. Scientists take thin tissue slices mounted on conductive microscope slides and apply a suitable MALDI matrix to the tissue, either manually or automatically. Next, the microscope slide is inserted into a MALDI mass spectrometer. The mass spectrometer records the spatial distribution of molecular species such as peptides, proteins or small molecules. Suitable image processing software can be used to import data from the mass spectrometer to allow visualization and comparison with the optical image of the sample. Recent work has also demonstrated the capacity to create three-dimensional molecular images using MALDI imaging technology and comparison of these image volumes to other imaging modalities such as magnetic resonance imaging (MRI).

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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